All pulled ESI emitters are made of quartz silica. For optimum performance, the inner surface is rinsed with acidified water, but you will need to condition it for your set-up. This will happen automatically in the first two runs of your nLC workflow. When you install a new emitter, we recommend you to pass two standard runs before analyzing your samples.
The Emitter surface finish is polished and rinsed with acidified water (HPLC grade). Under the influence of the electric field, the liquid wets the glass and the meniscus is anchored at the outer edge of the tip. The typical voltages required to stabilize the emitters is 1.5kV to 2.5kV, smaller orifice requires lower voltage.
Fresh Bioscience Ltd. offers two types of integrated emitter fused silica capillary tubing, fritted and non-fritted. All tubing are designed for user to self pack their own nanoLC columns with high performance and reproducibility. Normally we recommend fritted integrated emitter fused silica capillary tubing for peptides mapping, it drives the highest proteomics performance available. A consistent results over multiple experiments and hundreds of injections, which always make you be happy.
